GFP anticorps (HRP)

N° du produit ABIN297131

L’anticorps Chèvre Polyclonal anti-GFP a été validé pour WB, ELISA, IHC et IF. Il convient pour détecter GFP dans des échantillons de Aequorea victoria.

Aperçu rapide pour GFP anticorps (HRP) (ABIN297131)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-GFP anticorps (HRP)

Specificité: Recognizes wild type, recombinant and enhanced GFP. Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against human, mouse and rat serum proteins.

Purification: Immunoaffinity purified

Immunogène: GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (1:10000 - 1:50000), IF, IHC (1:500 - 1:3000), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA: 1:10000-1:50000. Sandwich or capture. Recognizes wild type, recombinant and enhanced forms of GFP. Immunohistochemistry: 1:500-1:3000. Immunofluorescence: Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic. (E. coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Western Blot: 1:1000-1:5000. Detects a 42kD band. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212